Journal: Genes & Cancer

Article Title: Requirement for BUB1B/BUBR1 in tumor progression of lung adenocarcinoma

doi:

Figure Lengend Snippet: A. Soft agar colony formation of mouse lung adenocarcinoma cell lines transfected with siNTC or mouse Bub1b siRNA SMARTpool. Colony numbers were shown as relative values normalized to siNTC controls. Mean±SEM was calculated from two independent experiments. The t-test p-values for siNTC vs siBub1b in LKR13 and LKR13_shTP53 cells were 0.09 and 0.07, respectively. B. BUB1B protein levels in LKPH2 cells expressing inducible shNTC or shBub1b 72 hours after induction with 100ng/ml doxycycline. C. Soft agar colony formation of LKPH2 cells from B. Colony numbers were shown as relative values normalized to no doxycycline treatment controls. Mean±SEM was calculated from three independent experiments. D. Cell proliferation of LKPH2 cells from B measured by CellTiterGlo. Values were normalized to no doxycycline treatment controls. Mean±SEM was calculated from two independent experiments. E. Soft agar colony formation of human lung adenocarcinoma cell lines transfected with siNTC or human BUB1B siRNA SMARTpool. Colony numbers were shown as relative values normalized to siNTC controls. Mean±SEM was calculated from two (HCC827, H23 and H460) to three independent experiments. The t-test p-values for siNTC vs siBub1b in HCC827, H23 and H460 cells were 0.1, 0.09 and 0.07, respectively. WT/wt, wild type. MT/mt, mutant. F and G. Cell lines in E were grouped based on their KRAS (F) or TP53 (G) status. *** indicates p<0.001, ** p<0.01, * p<0.05.

Article Snippet: Human lung adenocarcinoma cell lines were obtained from the Genentech Cell Line Repository.

Techniques: Transfection, Expressing, Mutagenesis

Journal: Genes & Cancer

Article Title: Requirement for BUB1B/BUBR1 in tumor progression of lung adenocarcinoma

doi:

Figure Lengend Snippet: A. BUB1B mRNA levels in multiple cancer types and their corresponding normal tissues from TCGA. nRPKM expression levels are shown in the boxplot on a log2-transformed scale, with display of the median expression level. The box represents 25-75% percentile. The fold-change between the mean tumor and the mean normal tissue expression is shown in the barplot on a log2-transformed scale. Significance was determined using a two-tailed t-test, with green indicative of a significant up-regulation in tumor samples (p< 0.05). B and C. BUB1B expression versus tumor stage (B) or lymph node metastasis (C) in TCGA lung adenocarcinoma clinical samples. One-way ANOVA test followed by Tukey's multiple comparison test was performed to determine significance. ** indicates p<0.01, * p<0.05. D. Kaplan-Meier plot for overall survival in TCGA lung adenocarcinoma clinical samples, with patients stratified into BUB1B high ( BUB1B expression above median; red solid line) and BUB1B low ( BUB1B expression below median; blue dotted line). CI, confidence interval.

Article Snippet: Human lung adenocarcinoma cell lines were obtained from the Genentech Cell Line Repository.

Techniques: Expressing, Transformation Assay, Two Tailed Test, Comparison

Journal: Scientific Reports

Article Title: Implementation and Quality Control of Lung Cancer EGFR Genetic Testing by MALDI-TOF Mass Spectrometry in Taiwan Clinical Practice

doi: 10.1038/srep30944

Figure Lengend Snippet: Accuracy Test of MALDI-TOF MS in EGFR Mutation Testing by Traceable Biological Materials.

Article Snippet: PC9 , Human lung adenocarcinoma cell line , RIKEN BioResource Center , Del19 , No , 0.0 , No , 0.3 , Yes , 99.2 , RIKEN BioResource ID: RCB4455.

Techniques: Mutagenesis, Plasmid Preparation, Clone Assay, Expressing